A proposal of multiplace hyperbaric chamber for animal experimentation and veterinary use.
Rech FV, Fagundes DJ, Hermanson R, Rivoire HC, Fagundes AL.
Integrate Regional University of Alto Uruguai and Missoes, Erechim, RS, Brazil.
Acta Cir Bras. 2008 Aug;23(4):384- 390.
PURPOSE: To develop a project of hyperbaric chamber that allows its safe and reliable use in veterinary and animal experimentation.
METHODS: Based on the technical specifications for the construction of hyperbaric chambers for human beings, it has been developed a design of a chamber with dimensions and characteristics for the use of a midsize animal, (dog or pig), as well as a multiple chamber for the use in small animals (mice, rats, hamsters, rabbits or cats). The technical specifications allowed that the chamber could be used both for veterinary use and for use in experiments on Health Sciences.
RESULTS: A chamber with the following characteristics was built: ASTM A36 steel for the manufacture of the master cylinder and rear cover; front door built in 5052 aluminum; internal diameter of 50.5 cm and 83.0 cm in length; weight 160Kg and internal area of 150cm(3); internal space to accommodate 2 acrylic baskets; 150mm high, 280mm wide and 690mm in length. It was capable of supporting a maximum of hydrostatic pressure test of 3.0 to 4.0 BAR ACT and maximum working pressure of 2.0 BAR or 3.0 ACT; equipped with security devices and valves that triggers with load of 2.2 BAR or 3.2 ACT. Tests for engineering and biological use on animals showed the effectiveness of the device.
CONCLUSION: The development of the project enabled the construction of a hyperbaric chamber with security features and reliability comparable to those required by the legal and technical specifications of a hyperbaric chamber human use.
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Barotraumatic orbital emphysema of rhinogenic origin in a breath-hold diver: a case report.
Bolognini A, Delehaye E, Cau M, Cosso L.
Sardinian Institute of Hyperbaric and Subaquatic Medicine, Sassari, Italy.
Undersea Hyperb Med. 2008 May-Jun;35(3) :163-7.
Orbital emphysema is a well-recognized complication of fractures involving the orbit. Commonly, it occurs when high pressure develops in nasal cavity as during nose blowing, coughing or Valsalva’s maneuver and usually occurs in the subcutaneous tissues. We report the case of a young breath-hold diver who developed spontaneous, non compressive orbital emphysema during underwater fishing, with a maximal depth of 25-30 meters in the Sardinian sea. He was otherwise healthy, without previous cranio-facial trauma and nasosinusal diseases or surgery were not present in the history. When he was referred to our attention the patient presented right eyelid ptosis but diplopia and vision impairment were absent. Computer tomography scans showed subcutaneous air in the right upper eyelid and around the eyeball, particularly near the orbit’s roof but optic nerve area, intraconal, was free of air. A dehiscence in lamina papyracea was evident. In our opinion, this has been the point of air entry into the orbit. A supportive therapy was advised and two weeks later the emphysema was recovered completely and the subject was symptoms free. The literature has been revised and to our knowledge no previous cases of barotraumatic orbital emphysema, in a breath-hold diver, are referred.
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Bioengineered alternative tissues and hyperbaric oxygen in lower extremity wound healing.
MED 08-42 200717613390 NDN- 230-0824-2194-5
AUTHORS- Kim, Paul J; Heilala, Matt; Steinberg, John S; Weinraub, Glenn M
JOURNAL NAME- Clin Podiatr Med Surg
VOLUME 24
NUMBER 3
PUBLICATION DATE- 2007 Jul
PP 529-46, x
DOCUMENT TYPE- Journal Article
JOURNAL CODE- 8604974
JOURNAL SUBSET- MEDJSIM
ISSN- 0891-8422
CORPORATE AUTHOR- Foot and Ankle Institute, 918 Congress Avenue Ste 200, Austin, TX 78701, USA. pkimxx@midwestern.edu
PUBLICATION COUNTRY- United States
LANGUAGE- English
With the advent and clinical application of recombinant chemical and cellular mediators of wound healing and a better understanding of the importance of serial debridement, most foot wounds can be healed with little morbidity. Despite these advances, there remains the recalcitrant wound for which more heroic efforts seem warranted. For these patients, advanced wound healing technologies, orthobiologics, and bioengineered alternative tissues may tilt the scales in the direction of definitive wound closure.
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Cerebral air embolism treated with hyperbaric oxygen therapy following percutaneous transthoracic computed tomography-guided needle biopsy of the lung.
MED 08-44 200818677615 NDN- 230-0830-9018-6
AUTHORS- Tomabechi, Makiko; Kato, Kenichi; Sone, Miyuki; Ehara, Shigeru; Sekimura, Kenshi; Kizawa, Tetsuya; Kin, Masakado
JOURNAL NAME- Radiat Med
VOLUME 26
NUMBER 6
PUBLICATION DATE- 2008 Jul
PP 379-83
DOCUMENT TYPE- Journal Article
JOURNAL CODE- 8412264
JOURNAL SUBSET- MEDJSIM
ISSN- 0288-2043
CORPORATE AUTHOR- Department of Radiology, Iwate Medical University School of Medicine, 19-1 Uchimaru, Morioka 020-8505, Japan. mtomabec@iwate-med.ac.jp
PUBLICATION COUNTRY- Japan
LANGUAGE- English
A 71-year-old man presented with cough and sputum for 12 months. Chest radiography showed a homogeneous opacity in the right lower lobe. Computed tomography (CT) showed a nodular opacity, 2 cm in diameter, in the posterior segment of the right lower lobe. Mild emphysematous changes were also seen. With the patient in a prone position, a 19-gauge 7.8-cm introducer was placed in the lesion during a single inspiratory breath-hold. A coaxial 20-gauge automated needle was inserted through the introducer using a biopsy gun. Although the patient did not complain of any symptoms, postbiopsy CT showed air in the left ventricle and ascending aorta. After 5 h of bed rest, we found weakness in his left lower extremity. He was transferred to a hyperbaric oxygen chamber and recovered the next day. Air embolism is a rare, potentially fatal complication of percutaneous lung biopsy. Although the true effect of hyperbaric oxygen therapy is controversial, knowledge regarding the prompt management of such cases may help radiologists who perform this procedure.
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Effect of hyperbaric oxygen on acute graft-versus-host disease after allogeneic bone marrow transplantation.
MED 08-44 200818549642 NDN- 230-0830-8086-4
AUTHORS- Song, Xiao-Yu; Sun, Lu-Ning; Zheng, Ning-Ning; Zhang, Hai-Peng
JOURNAL NAME- Zhongguo Shi Yan Xue Ye Xue Za Zhi
VOLUME 16
NUMBER 3
PUBLICATION DATE- 2008 Jun
PP 623-6
DOCUMENT TYPE- Journal Article
JOURNAL CODE- 101084424
JOURNAL SUBSET- MEDJSIM
ISSN- 1009-2137
CORPORATE AUTHOR- Department of Pathophysiology, College of Basic Medical Sciences, China Medical University, Shenyang 110001, Liaoning Province, China.
PUBLICATION COUNTRY- China
LANGUAGE- English
The objective of this study was to investigate the function and mechanism of hyperbaric oxygen (HBO) in antagonizing acute graft-versus-host disease (aGVHD) and improving the rate of survival. The lethally irradiated C57BL/6 recipients were injected with bone marrow and lymphocyte of spleen from BALB/c donors and were treated with HBO, cyclosporine A (CsA) and methotrexate (MTX). T lymphocytes and subsets, adhesion molecules and cytokines were detected by flow cytometry, ELISA and RT-PCR respectively. The results showed that the survival rate in HBO group was much higher than that in allogenetic bone marrow transplantation (allo-BMT) group and CsA + MTX group; the numbers of CD3(+), CD4(+), CD8(+), CD4(+)CD11a(+), CD4(+)CD18(+), CD8(+)CD11a(+), CD8(+)CD18(+) lymphocytes in spleen were decreased markedly by HBO and CsA + MTX (p < 0.05); the levels of IL-2 and TNFalpha mRNA and their serum concentrations in HBO group were much lower than those in allo-BMT group but were higher than those in CsA + MTX group; the levels of IL-4 and IL-10 mRNA in HBO group were much higher than those in allo-BMT group and CsA + MTX group. It is concluded that HBO has more remarkable advantage in improving the rate of survival than CsA + MTX, its mechanism of anti-aGVHD is tightly correlated with the transform of T cell and its subsets and the expression of adhesion molecules and cytokines.
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Effect of hyperbaric oxygen on patients with traumatic brain injury.
Lin JW, Tsai JT, Lee LM, Lin CM, Hung CC, Hung KS, Chen WY, Wei L, Ko CP, Su YK, Chiu WT.
Department of Neurosurgery, Taipei Medical University-Wan Fang Hospital, Taipei, Taiwan.
Acta Neurochir Suppl. 2008;101:145- 9
Hyperbaric oxygen therapy (HBOT) is the medical therapeutic use of oxygen at a higher atmospheric pressure. The United States Food and Drug Administration have approved several clinical applications for HBOT, but HBOT in traumatic brain injury (TBI) patients has still remained in controversial. The purpose of our study is to evaluate the benefit of HBOT on the prognosis of subacute TBI patients. We prospectively enrolled 44 patients with TBI from November 1, 2004 to October 31, 2005. The study group randomly included 22 patients who received HBOT after the patients’ condition stabilization, and the other 22 corresponding condition patients were assigned into the matched control group who were not treated with HBOT. The clinical conditions of the patients were evaluated with the Glasgow Coma Scale (GCS) and Glasgow Outcome Scale (GOS) before and 3 to 6 months after HBOT. The GCS of the HBOT group was improved from 11.1 to 13.5 in average, and from 10.4 to 11.5 (p < 0.05) for control group. Among those patients with GOS = 4 before the HBOT, significant GOS improvement was observed in the HBOT group 6 months after HBOT. Based on this study, HBOT can provide some benefits for the subacute TBI patients with minimal adverse side effects.
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Effects of hyperbaric oxygen therapy on facial nerve regeneration.
Vilela DS, Lazarini PR, Da Silva CF.
Otorhinolaryngology Department, Santa Casa Sao Paulo, Faculty of Medical Sciences, Sao Paulo, Brazil.
Acta Otolaryngol. 2008 Mar 10;:1-5 [Epub ahead of print]
Conclusion. Hyperbaric oxygen treatment (HBOT) promoted an increase of the mean axonal diameter in the group evaluated 2 weeks after lesion induction, which suggests a more advanced regeneration process. However, the number of myelin nerve fibers of the facial nerve of the rabbits was similar when compared to the control and treatment groups, in both evaluation periods.
Objective. To evaluate the effect of HBOT on the histological pattern of the facial nerve in rabbits exposed to a nerve crush injury. Materials and methods. Twenty rabbits were exposed to facial nerve crush injury. Ten rabbits received HBOT, 10 rabbits comprised the control group. The rabbits were sacrificed 2 and 4 weeks after the trauma. Qualitative morphological analysis, measurement of the external axonal diameters and myelin fiber count were carried out in an area of 185 000 microm(2). Results. There was an increase in the area of the axons and thicker myelin in the 2 weeks treatment group in comparison with the control group. The mean diameter of the axons was of 2.34 microm in the control group and of 2.81 microm in the HBOT group, with statistically significant differences. The 2 week control group had a mean number of myelin fibers of 1865.2+/-664, and the HBOT group had a mean number of 2026.3+/-302; this was not statistically significant. The 4 week control group presented a mean of 2495.1+/-479 fibers and the HBOT group presented a mean of 2359.9+/-473; this was not statistically significant.
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Effects of Hyperbaric Oxygen Therapy on Wound Healing After Tracheal Resection and End-to-End Anastomoses in Rats: Results of Early Observations.
MED 08-43 200818704859 NDN- 230-0829-2644-8
AUTHORS- Gorur, R; Hahoglu, A; Uzun, G; Kutlu, A; Turut, H; Yiyit, N; Candas, F; Isitmangil, T
JOURNAL NAME- Thorac Cardiovasc Surg
VOLUME 56
NUMBER 6
PUBLICATION DATE- 2008 Sep
PP 359-62
DOCUMENT TYPE- Journal Article
JOURNAL CODE- 7903387
JOURNAL SUBSET- MEDJSIM
ISSN- 0171-6425
CORPORATE AUTHOR- Department of Thoracic Surgery, GATA Haydarpasa Teaching Hospital, Istanbul, Turkey.
PUBLICATION COUNTRY- Germany
LANGUAGE- English
BACKGROUND: Proliferating scar tissue is an important problem after tracheal surgery. Hyperbaric oxygen (HBO) provides good support to the poorly perfused tissues. We aimed to evaluate the early effects of HBO therapy on tracheal healing after tracheal resection.
METHODS: A total of 15 Wistar rats were divided into two groups and two tracheal rings of each rat were resected. A control group received no therapy while the other group was treated with HBO. At the end of the study or when a rat died, a histopathological examination was performed to assess neovascularization, necrosis and epithelization.
RESULTS: HBO treatment caused better epithelization and inflammation scores compared to the control group. In the HBO group the intraluminal purulent exudates were limited ( P = 0.001). Healing of the damaged mucosal epithelium was better in the HBO group ( P = 0.031). In the HBO-treated group, neovascularization also started earlier than in the group without HBO. CONCLUSION: Our short-term observation results demonstrate that HBO treatment increases tracheal healing and decreases the complication ratios. HBO may be preferred as a supportive treatment modality during the healing period after tracheal surgery.
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Effects of pressure, cold and gloves on hand skin temperature and manual performance of divers.
MED 08-44 200818369658 NDN- 230-0830-6562-6
AUTHORS- Zander, Joanna; Morrison, James
JOURNAL NAME- Eur J Appl Physiol
VOLUME 104
NUMBER 2
PUBLICATION DATE- 2008 Sep
PP 237-44
DOCUMENT TYPE- Journal Article
JOURNAL CODE- 100954790
JOURNAL SUBSET- MEDJSIM
ISSN- 1439-6319
CORPORATE AUTHOR- School of Kinesiology, Simon Fraser University, 8888 University Drive, Burnaby, BC, V5A 1S6, Canada.
PUBLICATION COUNTRY- Germany
LANGUAGE- English
Cold water immersion and protective gloves are associated with decreased manual performance. Although neoprene gloves slow hand cooling, there is little information on whether they provide sufficient protection when diving in cold water. Nine divers wearing three-fingered neoprene gloves and dry suits were immersed in water at 25 and 4 degrees C, at depths of 0.4 msw (101 kPa altitude adjusted) and 40 msw (497 kPa) in a hyperbaric chamber Skin temperatures were measured at the fingers, hand, forearm, chest and head. Grip strength, tactile sensitivity and manual dexterity were measured at three time intervals. There was an exponential decay in finger and back of hand skin temperatures with exposure time in 4 degrees C water. Finger and back of hand skin temperatures were lower at 40 msw than at 0.4 msw (P < 0.05). There was no effect of pressure or temperature on grip strength. Tactile sensitivity decreased linearly with finger skin temperature at both pressures. Manual dexterity was not affected by finger skin temperature at 0.4 msw, but decreased with fall in finger skin temperature at 40 msw. Results show that neoprene gloves do not provide adequate thermal protection in 4 degrees C water and that impairment of manual performance is dependent on the type of task, depth and exposure time.
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Hyperbaric oxygen therapy aggravates liver reperfusion injury in rats.
Lima CX, Sanches MD, Rezende Neto JB, Silva RC, Teixeira MM, Souza DD, Santos GD, Melo JR.
Department of Physiology, School of Science, Federal University of Minas Gerais, Brazil.
Acta Cir Bras. 2008 Aug;23(4):315- 321
PURPOSE: To evaluate the effects of hyperbaric oxygen (HO) therapy in the protection against liver ischemia/reperfusion injury.
METHODS: Thirty-two male Wistar rats were divided into four groups of eight animals each: group A - laparotomy and liver manipulation, group B - liver ischemia and reperfusion, group C - HO pretreatment for 60 min followed by liver ischemia and reperfusion, and group D - pretreatment with ambient air at 2.5 absolute atmospheres for 60 min followed by liver ischemia and reperfusion. Plasma was assayed for aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH). Intra-arterial blood pressure was monitored continuously. Myeloperoxidase activity in the liver and lung was assessed 30 min after reperfusion.
RESULTS: Plasma AST, ALT and LDH increased after reperfusion in all animals. Plasma ALT values and myeloperoxidase activity in the liver parenchyma were higher in HO-pretreated animals than in groups A, B and D. HO had a negative hemodynamic effect during liver reperfusion.
CONCLUSION: Liver preconditioning with hyperbaric oxygen therapy aggravated liver ischemia/reperfusion injury in rats as demonstrated by plasma ALT and liver myeloperoxidase activity.
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Hyperbaric oxygen therapy for radiation-induced hemorrhagic cystitis.
Yoshida T, Kawashima A, Ujike T, Uemura M, Nishimura K, Miyoshi S.
The Department of Urology, Osaka Rosai Hospital, and The Department of Urology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan.
Int J Urol. 2008 Jul;15(7):639- 41
Hyperbaric oxygen (HBO) therapy has recently emerged as a potential primary option for the management of hemorrhagic cystitis. We review our experience treating hemorrhagic cystitis with HBO. Between January 2001 and May 2007, eight patients with radiation-induced hemorrhagic cystitis underwent HBO therapy. There were five men and three women with a mean age of 64.3 years (47-73). Radiation was given for local disease, and the mean dosage delivered was 56.6 Gy (42-70). The mean duration between the onset of hematuria and the beginning of HBO therapy was 8.9 months (3-34). Mean follow-up period was 15.5 months (2-31). Hematuria resolved completely in six of the eight patients, one of whom suffered recurrence of hematuria and was treated with HBO until the hematuria resolved again. The response rate was 75%, compatible with the previous reports, and no side-effects of HBO were noted. HBO treatment should be attempted for radiation-induced hemorrhagic cystitis.
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Hyperbaric oxygenation for tumour sensitisation to radiotherapy: A systematic review of randomised controlled trials.
Bennett M, Feldmeier J, Smee R, Milross C.
Department of Diving and Hyperbaric Medicine, Prince of Wales Hospital, University of NSW, Randwick, NSW 2031, Australia.
Cancer Treat Rev. 2008 Jul 18;
BACKGROUND: Radiotherapy is a well-established treatment for some solid tumours. Hyperbaric oxygenation (HBO) may improve radiotherapeutic killing of hypoxic cancer cells, so the simultaneous administration of radiotherapy and HBO may reduce mortality and tumour recurrence.
METHODS: We performed a systematic search of the literature in September 2007 for randomised controlled trials, and made pooled analyses of pre-determined clinical outcomes.
RESULTS: Nineteen trials contributed to this review (2286 patients). There was a reduction in mortality for head and neck cancers at one and five years after therapy (at five years RR 0.82, P=0.03, NNT=5), and improved local tumour control at three months (RR 0.58, P=0.006, NNT=7). Any advantage is achieved at the cost of an increased rate of both severe radiation tissue injury (RR 2.35, P<0.0001, NNH=8) and the chance of seizures during therapy (RR 6.76, P=0.03, NNH=22).
CONCLUSIONS: There is some evidence that HBO improves local tumour control and mortality for cancers of the head and neck, and local tumour recurrence in cancers of the uterine cervix. These benefits may only occur with unusual fractionation schemes. HBO is associated with significant adverse effects including oxygen toxic seizures and severe radiation tissue injury. The methodological and reporting inadequacies of the studies included in this review demand a cautious interpretation. More research is needed for head, neck and uterine cervical cancer, but is probably not justified for bladder cancer. There is little evidence available concerning malignancies at other sites.
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Hyperbaric oxygen therapy in BKV-associated hemorrhagic cystitis refractory to intravenous and intravesical cidofovir: Case report and review of literature.
Focosi D, Maggi F, Pistolesi D, Benedetti E, Papineschi F, Galimberti S, Ceccherini-Nelli L, Petrini M.
Division of Hematology, Department of Oncology, Transplantations and New Technologies in Medicine, University of Pisa, Italy.
Leuk Res. 2008 Jul 23; [Epub ahead of print]
Hemorrhagic cystitis is a common complication in hematopoietic stem cell transplant recipients. We report here a case of severe BKV-associated hemorrhagic cystitis who did not respond to intravenous cidofovir. Overt hematuria successfully resolved after a few days on hyperbaric oxygen and intravesical instillations of cidofovir, while BK viruria dropped after a few weeks and remained low. We review the literature for therapeutic options in hemorrhagic cystitis and try to explain how hyperbaric oxygen stimulates mucosal repair in the urinary bladder.
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Hyperbaric oxygen preconditioning promotes angiogenesis in rat liver after partial hepatectomy.
Ren P, Kang Z, Gu G, Liu Y, Xu W, Tao H, Zhang JH, Sun X, Ji H.
Department of Pharmacology, China Pharmaceutical University, Nanjing, 210009, PR China.
Life Sci. 2008 Jun 24; [Epub ahead of print]
Hyperbaric oxygen preconditioning (HBO-PC) increases the level of HIF-1alpha (hypoxia inducible factor-1alpha) and its target gene VEGF (vascular endothelial growth factor) which is involved in angiogenesis. Liver regeneration is an angiogenesis- dependent process. We hypothesized that HIF-1alpha and VEGF mediated the angiogenesis effect of HBO-PC on regenerating rat liver. Male Sprague Dawley rats received HBO-PC followed by 70% partial hepatectomy. Proliferation of hepatocytes and endothelial cells was evaluated by BrdU (bromodeoxyuridine) staining. Microvascular density was assessed by immunohistochemistr y. mRNA expression of HIF-1alpha was assessed by quantitative RT-PCR and protein levels of HIF-1alpha and VEGF were assessed by western blot. HIF-1alpha DNA-binding activity was determined with an ELISA-based kit. HBO-PC increased the proliferation index of endothelial cells and microvascular density at 48 h after partial hepatectomy. The protein level and DNA-binding activity of HIF-1alpha and the protein level of VEGF were increased by HBO-PC before and after partial hepatectomy. Partial hepatectomy alone also increased proliferation index and the expressions of HIF-1alpha and VEGF. Our results indicated that the angiogenesis effect of HBO-PC on liver after partial hepatectomy could be achieved by increased HIF-1alpha activity and VEGF expression. However, the angiogenic effect of HBO-PC is moderate and HBO-PC failed to produce additional effect on the enhancement of HIF-1alpha and VEGF induced by partial hepatectomy alone.
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Hyperbaric oxygen therapy seems to enhance recovery from acute acoustic trauma.
Ylikoski J, Mrena R, Makitie A, Kuokkanen J, Pirvola U, Savolainen S.
Helsinki Ear Institute and Department of Otolaryngology and Institute of Biotechnology, University of Helsinki, and Centre for Military Medicine, Helsinki, Finland.
Acta Otolaryngol. 2008 Apr 1;:1-7 [Epub ahead of print]
Conclusion. The average recovery of hearing and cessation of tinnitus was significantly better after hyperbaric oxygen therapy (HBOT) than after normobaric oxygen therapy (NBOT). HBOT can be valuable adjuvant therapy for patients with acute acoustic trauma (AAT). Objectives. AAT was one of the early indications for the use of HBOT. The rationale of administering oxygen to patients with AAT is based on experimental studies showing that noise exposure results in cochlear hypoxia, which could be compensated by HBOT. The aim of this study was to investigate the efficacy of HBOT in patients with AAT. Patients and methods. We compared the recovery from hearing impairment and tinnitus in 60 ears treated with HBOT with 60 ears treated with NBOT. The HBOT was given daily for 1-8 days. There were no significant differences in clinical or audiological data between HBOT and NBOT groups. Results. The average recovery of hearing both at high and speech frequencies was significantly better and tinnitus persisted less commonly after the HBOT than after the NBOT. Normal hearing at the end of the follow-up period was regained in 42 ears in the HBOT group and in 24 ears in the NBOT group (p<0.01).
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Hyperbaric oxygen therapy as a prophylactic and treatment against ileus and recurrent intestinal obstruction soon after surgery to relieve adhesive intestinal obstruction.
MED 08-43 200817593223 NDN- 230-0828-2854-5
AUTHORS- Ambiru, Satoshi; Furuyama, Nobuaki; Kimura, Fumio; Shimizu, Hiroaki; Yoshidome, Hiroyuki; Miyazaki, Masaru; Shimada, Hideaki; Ochiai, Takenori
JOURNAL NAME- J Gastroenterol Hepatol
VOLUME 23
NUMBER 8 Pt 2
PUBLICATION DATE- 2008 Aug
PP e379-83
DOCUMENT TYPE- Journal Article
JOURNAL CODE- 8607909
JOURNAL SUBSET- MEDJSIM
ISSN- 1440-1746
CORPORATE AUTHOR- Departments of General Surgery, Surgical Center, Chiba University Hospital, Chiba University Graduate School of Medicine, Chiba, Japan. ambiru-s@umin.ac.jp
PUBLICATION COUNTRY- Australia
LANGUAGE- English
BACKGROUND AND AIM: Nonoperative management of cases of adhesive intestinal obstruction would be ideal, especially for patients who have recently undergone surgery to relieve the same condition. We aimed to examine whether hyperbaric oxygen (HBO) therapy might have therapeutic potential for the treatment of postoperative paralytic ileus and recurrent adhesive intestinal obstruction soon after surgery, to relieve adhesive intestinal obstruction, because of its unique mechanisms in these contexts.
METHODS: A total of 133 patients were enrolled in the present study. We examined non-per os periods, hospital stay, and clinical course according to the postoperative course of the 133 patients. RESULTS: After surgical intervention, 75 patients left the hospital without morbidity. Nineteen patients were successfully administered prophylactic HBO therapy to facilitate intestinal motility and to prevent paralytic ileus. The remaining 39 patients suffered from postoperative paralytic ileus or early recurrence of obstruction during the same hospitalization period. The patients who underwent prophylactic HBO therapy had significantly shorter non-per os periods and hospital stays after surgery than those who were not initially given HBO therapy (P < 0.05). Similarly, there were significant differences in duration of hospital stay after surgery between patients with HBO therapy as treatment and those who received other conservative therapies (P < 0.05).
CONCLUSIONS: HBO therapy may have a prophylactic effect on postoperative paralytic ileus and may be of therapeutic benefit in the management of early recurrent adhesive intestinal obstruction following surgery to relieve adhesive intestinal obstruction.
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Hyperbaric oxygen induces placental growth factor expression in bone marrow-derived mesenchymal stem cells.
MED 08-44 200818558410 NDN- 230-0830-8270-4
AUTHORS- Shyu, Kou-Gi; Hung, Huei-Fong; Wang, Bao-Wei; Chang, Hang
JOURNAL NAME- Life Sci
VOLUME 83
NUMBER 1-2
PUBLICATION DATE- 2008 Jul 4
PP 65-73
DOCUMENT TYPE- Journal Article; Research Support, Non-U.S. Gov’t
JOURNAL CODE- 0375521
JOURNAL SUBSET- MEDJSIM
ISSN- 0024-3205
CORPORATE AUTHOR- Division of Cardiology, Shin Kong Wu Ho-Su Memorial Hospital, and Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
PUBLICATION COUNTRY- England
LANGUAGE- English
The bone marrow is home to mesenchymal stem cells (MSCs) that are able to differentiate into many different cell types. The effect of hyperbaric oxygen (HBO) on MSCs is poorly understood. Placental growth factor (PlGF) is an attractive therapeutic agent for stimulating revascularization of ischemic tissue. HBO has been shown to improve diabetic wound healing by increase circulating stem cells. We hypothesized that HBO induces PlGF expression in bone marrow-derived MSCs. The MSCs were obtained from adult human bone marrow and expanded in vitro. The purity and characteristics of MSCs were identified by flow cytometry and immunophenotyping. HBO at 2.5 ATA (atmosphere absolute) significantly increased PlGF protein and mRNA expression. The induction of PlGF protein by HBO was significantly blocked by the addition of N-acetylcysteine, while wortmannin, PD98059, SP600125 and SB203580 had no effect on PlGF protein expression. However, the specific inhibitor of nitric oxide synthase, L-NAME did not alter the PlGF protein expression induced by HBO. HBO significantly increased the reactive oxygen species production and pretreatment with N-acetylcysteine significantly blocked the induction of reactive oxygen species by HBO. HBO significantly increased the migration and tube formation of MSCs and pretreatment with N-acetylcysteine and PlGF siRNA significantly blocked the induction of migration and tube formation by HBO. In conclusion, HBO induced the expression of PlGF in human bone marrow-derived MSCs at least through the oxidative stress-related pathways, which may play an important role in HBO-induced vasculogenesis.
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Hyperbaric oxygen preconditioning promotes angiogenesis in rat liver after partial hepatectomy.
MED 08-43 200818644387 NDN- 230-0827-2562-6
AUTHORS- Ren, Ping; Kang, Zhiming; Gu, Guojun; Liu, Yun; Xu, Weigang; Tao, Hengyi; Zhang, John H; Sun, Xuejun; Ji, Hui
JOURNAL NAME- Life Sci
VOLUME 83
NUMBER 7-8
PUBLICATION DATE- 2008 Aug 15
PP 236-41
DOCUMENT TYPE- Journal Article
JOURNAL CODE- 0375521
JOURNAL SUBSET- MEDJSIM
ISSN- 0024-3205
CORPORATE AUTHOR- Department of Pharmacology, China Pharmaceutical University, Nanjing, 210009, PR China.
PUBLICATION COUNTRY- England
LANGUAGE- English
Hyperbaric oxygen preconditioning (HBO-PC) increases the level of HIF-1alpha (hypoxia inducible factor-1alpha) and its target gene VEGF (vascular endothelial growth factor) which is involved in angiogenesis. Liver regeneration is an angiogenesis-dependent process. We hypothesized that HIF-1alpha and VEGF mediated the angiogenesis effect of HBO-PC on regenerating rat liver. Male Sprague Dawley rats received HBO-PC followed by 70% partial hepatectomy. Proliferation of hepatocytes and endothelial cells was evaluated by BrdU (bromodeoxyuridine) staining. Microvascular density was assessed by immunohistochemistry. mRNA expression of HIF-1alpha was assessed by quantitative RT-PCR and protein levels of HIF-1alpha and VEGF were assessed by western blot. HIF-1alpha DNA-binding activity was determined with an ELISA-based kit. HBO-PC increased the proliferation index of endothelial cells and microvascular density at 48 h after partial hepatectomy. The protein level and DNA-binding activity of HIF-1alpha and the protein level of VEGF were increased by HBO-PC before and after partial hepatectomy. Partial hepatectomy alone also increased proliferation index and the expressions of HIF-1alpha and VEGF. Our results indicated that the angiogenesis effect of HBO-PC on liver after partial hepatectomy could be achieved by increased HIF-1alpha activity and VEGF expression. However, the angiogenic effect of HBO-PC is moderate and HBO-PC failed to produce additional effect on the enhancement of HIF-1alpha and VEGF induced by partial hepatectomy alone.
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